In liquid chromatography analysis, we make the following summary about the replacement content of positive and reversed phase chromatography
1. First, use n-hexane (silica gel column) to equilibrate the chromatography system including the normal phase column (guarantee that the baseline is straight and no peak appears within 20 minutes);
2. Remove the silica gel column, seal it and save;
3. Short-circuit the sampling valve and the detector, flush the chromatography system with isopropyl alcohol for 10 minutes (flow rate 1ml / min). During this period, empty the sampling valve three times;
4. Change the methanol (or eye) to flush the system for 20 minutes (flow rate 2ml / min), during this period empty the sampling valve three times;
5. Install the reversed-phase chromatography column, adjust the flow rate to 1ml / min, rinse the system with methanol (or eye) until the system is balanced;
6. Into the three-pin standard product, check whether the area and retention time are repeated, if not, continue to rinse until repeated.
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