Possible problems and treatment in the process of elisa experiment

Generally speaking, the failure of the experiment should be a technical error, equipment damage or reactant failure. When an experiment fails, check the expiration date of each reactant and make sure that all reactants are stored according to the instructions on the product label. In addition, check the contents of the notes. When suspicious phenomena or problems occur during the test, you can refer to the following table to analyze the problem.


Possible problem
possible reason
Adopt measures
No color
Missing enzyme-labeled antibody
Check the operation process, be careful not to miss
Enzyme-labeled antibodies contaminate sodium azide
The pollution of sodium azide should be strictly avoided
Mixing different kit components
Check the reagent label to confirm that all components belong to the kit
Weak color rendering
The product has expired
Check product expiration date
Wrong volume and time of reagent added
Confirm that the volume of each reagent used and the time of addition are in accordance with the instructions
Reagents and samples are not balanced before use
Reagents and samples are equilibrated at room temperature for 30 minutes
Insufficient incubation time
Check the incubation time
Incubate enzyme-labeled plates in a temperature-changing environment
Determine the incubation temperature to avoid temperature changes
The instrument settings are incorrect and the filters do not match
Whether the instrument is set correctly and the filter is used correctly
Contaminated reagents used
Check if the reagent is contaminated
Incomplete
After the clapboard, the board should dry
Whole board yellow
Buffer contamination
Prepare fresh buffer
Tip reuse
Tips should be used once
Equipment is contaminated
Non-polluting equipment should be used
The substrate liquid is contaminated
Substrate replacement
High complex aperture ratio
Incomplete washing
The washing part must be done thoroughly
Dry board appears, no sealed bag is used, the sealed bag is reused
The microplate should be kept moist. Incubate in a sealed bag. Pay attention to replace the new sealed bag at each step
Inadequate
After the clapboard, the board should dry
Unequal volume of liquid added in the well
Make sure that the pipette is calibrated and working properly, and the tip must not be reused
Buffer contamination
Prepare fresh buffer
High background
Buffer contamination
Prepare fresh buffer
Unequal volume of liquid added per well
Make sure the pipette is calibrated and working properly
Incomplete washing
The washing part must be done thoroughly
Inadequate
After the clapboard, the board should dry
Incubation time temperature and time are not appropriate in the experiment
Confirm whether the incubation temperature and time of each experimental step are appropriate
Enzyme-labeled antibody dilution error
Confirm the working concentration of enzyme-labeled antibody is configured correctly
Substrate liquid is exposed to light for a long time or contaminated
Substrate solution should be opened before use and avoid strong light
Tip reuse
Tips should be used once
Linear deviation of standard curve
Standard loading error
Make sure the pipette is calibrated and working properly
Enzyme-labeled antibody concentration is too low
Confirm the working concentration of enzyme-labeled antibody is configured correctly
Incubation time and temperature of enzyme plate color development are insufficient
Follow the instructions
Careless operation
Review the operation process and strictly follow the instructions


Possible problem
possible reason
Adopt measures
No color
Missing enzyme-labeled antibody
Check the operation process, be careful not to miss
Enzyme-labeled antibodies contaminate sodium azide
The pollution of sodium azide should be strictly avoided
Mixing different kit components
Check the reagent label to confirm that all components belong to the kit
Weak color rendering
The product has expired
Check product expiration date
Wrong volume and time of reagent added
Confirm that the volume of each reagent used and the time of addition are in accordance with the instructions
Reagents and samples are not balanced before use
Reagents and samples are equilibrated at room temperature for 30 minutes
Insufficient incubation time
Check the incubation time
Incubate enzyme-labeled plates in an environment with varying temperatures
Determine the incubation temperature to avoid temperature changes
The instrument settings are incorrect and the filters do not match
Whether the instrument is set correctly and the filter is used correctly
Contaminated reagents used
Check if the reagent is contaminated
Incomplete
After the clapboard, the board should dry
Whole board yellow
Buffer contamination
Prepare fresh buffer
Tip reuse
Tips should be used once
Equipment is contaminated
Non-polluting equipment should be used
The substrate liquid is contaminated
Substrate replacement
High complex aperture ratio
Incomplete washing
The washing part must be done thoroughly
Dry board appears, no sealed bag is used, the sealed bag is reused
The microplate should be kept moist. Incubate in a sealed bag. Pay attention to replace the new sealed bag at each step
Inadequate
After the clapboard, the board should dry
Unequal volume of liquid added in the well
Make sure that the pipette is calibrated and working properly, and the tip must not be reused
Buffer contamination
Prepare fresh buffer
High background
Buffer contamination
Prepare fresh buffer
Unequal volume of liquid added per well
Make sure the pipette is calibrated and working properly
Incomplete washing
The washing part must be done thoroughly
Inadequate
After the clapboard, the board should dry
Incubation time temperature and time are not appropriate in the experiment
Confirm whether the incubation temperature and time of each experimental step are appropriate
Enzyme-labeled antibody dilution error
Confirm the working concentration of enzyme-labeled antibody is configured correctly
Substrate liquid is exposed to light for a long time or contaminated
Substrate solution should be opened before use and avoid strong light
Tip reuse
Tips should be used once
Linear deviation of standard curve
Standard loading error
Make sure the pipette is calibrated and working properly
Enzyme-labeled antibody concentration is too low
Confirm the working concentration of enzyme-labeled antibody is configured correctly
Incubation time and temperature of enzyme plate color development are insufficient
Follow the instructions
Careless operation
Review the operation process and strictly follow the instructions

WHY THEMED INDOOR PLAYGROUNDS?
Our clients turn to themed playgrounds because these types of play spaces:

Work with any décor or business theme. Your business likely already has a color scheme and theme. Imagine how wonderful it would be to offer your clientele a playground space that matches your image. For example, if you have a nature museum, your themed playground can include butterflies and nature-themed equipment. If you have a car dealership and want to keep kids entertained while interacting with customers, you can create a car-themed playground.
Can be customized to suit any age group or space. Whether you have a large space or a limited amount of area for play, Golden Childhood can create a playground customized around your theme and your space. We can target the specific age range of your customers` children, helping you best serve your clientele and ensure the equipment is appropriate.
Can help boost the branding of your business. Imagine how exciting it would be for children and parents to see a playground which represents a major theme in your business. It can help you brand your business as a family-friendly enterprise. Themed playgrounds are more memorable for parents and children alike; imagine the photos families can take and post on social media as they play on your themed playground, spreading the word about your fun and family-friendly business!
Can help create an innovative, fun and memorable playground to meet your needs. Above all, Golden Childhood playgrounds are fun and unique. You`ll be able to offer children a great and memorable experience. Children won`t forget your playground when you build one just for them!

Indoor Playground Themes

Playground Themes,Indoor Playground Themes,Forest Themed Indoor Play Set,Themed Playground Structure

Shenyang Golden Childhood playground equipment CO.,LTD , https://www.outdoor-playground.com

Posted on